There is growing evidence that cellular metabolism can directly participate in epigenetic dynamics and consequently modulate gene expression.However,the role of metabolites in activating the key gene regulatory network for specialization of germ cell lineage remains largely unknown.Here,we identified some cellular metabolites with significant changes by untargeted metabolomics between mouse epiblast-like cells(EpiLCs) and primordial germ cell-like cells(PGCLCs).More importantly,we found that inhibition of glutaminolysis by bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide(BPTES) impeded PGCLC specialization,but the impediment could be rescued by addition of α-ketoglutarate(αKG),the intermediate metabolite of oxidative phosphorylation and glutaminolysis.Moreover,adding aKG alone to the PGCLC medium accelerated the PGCLC specialization through promoting H3 K27 me3 demethylation.Thus,our study reveals the importance of metabolite aKG in the germ cell fate determination and highlights the essential role of cellular metabolism in shaping the cell identities through epigenetic events.

文章来源：Cellular & Molecular Immunology 网址: http://cml.400nongye.com/lunwen/itemid-30102.shtml

上一篇： Endoscopic retrograde cholangiopancreatogra
下一篇： 消化系统疾病论文_肝受体同系物1对大鼠急性胰腺炎细胞模型中腺泡细胞损伤修复的影响及其机制